IN-VITRO EVALUATION OF ANTIOXIDATIVE, ANTIBACTERIAL, AND HEPATOPROTECTIVE EFFECT OF SMILAX ZEYLANICA L. AND BERBERIS ARISTATA DC

Antioxidant, Antibacterial & Hepatoprotective Potential of S. zeylanica and B. aristata

  • Manish Kumar Kaushik Research Scholar, I.T.S College of Pharmacy, Ghaziabad, Uttar Pradesh, India.
  • Monika Singh M.Pharm
  • Sadish Kumar Shanmugam Department of Pharmacy, I.T.S College of Pharmacy, Ghaziabad, Uttar Pradesh, India.
  • Nitin Kumar Department of Pharmacy, Meerut Institute of Technology, Meerut, Uttar Pradesh, India.
  • Moumita Barman Department of Pharmacy, I.T.S College of Pharmacy, Ghaziabad, Uttar Pradesh, India.

Abstract

Abstract


Background: The research examined the antioxidant, antibacterial, and hepatoprotective properties of hydroalcoholic extracts derived from Smilax zeylanica L. and Berberis aristata DC. Alkaloids, tannins, flavonoids, and glycosides were the major phytoconstituents, with a significant quantity of alkaloids and flavanones.


Objective: The hydroalcoholic extract of Smilax zeylanica exhibited an IC50 value of 6.29 μg/ml, while Berberis aristata demonstrated an IC50 value of 9.26 μg/ml in the DPPH (1,1-diphenyl-2- picrylhydrazyl) assay for antioxidant activity.


Methods: The modified extract showed significant antibacterial activity and Enterococcus faecalis exhibited significant susceptibility to the extract by using the disc diffusion method.


Results: The hepatoprotective activity of the extracts was assessed against HepG2 cells, and the extracts showed varying levels of hepatoprotection and cytotoxicity. Berberis aristata showed the highest hepatoprotective activity (IC50 < 47.09) among all the extracts.


Conclusion: From the above, it is suggested that natural antioxidants, antibacterials, and hepatoprotective properties of plant extracts of Smilax zeylanica and Berberis aristata could be promising for further research and can be used to develop effective antibacterial agents against microbial infections.

Keywords: Hepatoprotective, Antioxidant activity, Antibacterial activity, Cytotoxicity, Cell line

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How to Cite
Kaushik, M., M. Singh, S. Shanmugam, N. Kumar, and M. Barman. “IN-VITRO EVALUATION OF ANTIOXIDATIVE, ANTIBACTERIAL, AND HEPATOPROTECTIVE EFFECT OF SMILAX ZEYLANICA L. AND BERBERIS ARISTATA DC”. Journal of Applied Pharmaceutical Sciences and Research, Vol. 8, no. 01, May 2025, pp. 17-26, doi:10.31069/japsr.v8i1.03.
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Research Articles