DEVELOPMENT AND VALIDATION OF THE UV-SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF VIGNA RADIATA SEED EXTRACT
Abstract
Aim: The main goal was to develop and validate a sensitive, simple, accurate, precise, ruggedness and cost-effective UV spectrophotometric method for the estimation of Vigna radiata in prepared pharmaceutical formulations of smart lipid nanoparticles.
Methodology: The standard solution was prepared in water and a calibration curve was constructed after measuring absorbance. The different analytical performance parameters such as linearity, range, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness were determined according to International Conference on Harmonization (ICH) Q2 (R1) guidelines.
Results: Vigna radiata showed a maximum absorption at a wavelength of 281 nm. Beer-Lambert’s law was obeyed in the concentration range of 25-400 μg/ml with a correlation coefficient (R2) of 0.9968. The limit of detection and limit of quantification was found to be16.03μg/ml and 48.57μg/ml respectively. The precision and repeatability scores were all within acceptable limits. The recovery was found to be between 99.33% and 100.2% The precision and repeatability values were within a 2% tolerance range. Vigna radiata was found to have a purity of 99.33%
Conclusion: The study demonstrated that the developed procedure was accurate, precise and reproducible while being easy, environmentally friendly, repeatable, and cost-effective, and it can be used for quantification of Vigna radiata seed extract in pharmaceutical dosage forms.
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