DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR BERBERINE QUANTIFICATION
Abstract
Aim: The present work aimed to develop and validate a sensitive, simple, accurate, precise, ruggedness and cost-effective UV spectrophotometric method for the estimation of Berberine in prepared pharmaceutical formulations of smart lipid nanoparticles.
Methodology: The standard solution was prepared in methanol and a calibration curve was constructed after measuring absorbance. The different analytical performance parameters such as linearity, range, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) and robustness were determined according to International Conference on Harmonization (ICH) Q2 (R1) guidelines.
Results: Berberine showed a maximum absorption at a wavelength of 418 nm. Beer-Lambert’s law was obeyed in the concentration range of 1–15 μg/ml with a correlation coefficient (R2) of 0.9922. The limit of detection and limit of quantification was found to be1.565μg/ml and 4.742μg/ml respectively. The precision and repeatability scores were all within acceptable limits. The recovery was found to be between 99.64% and 100.39% The precision and repeatability values were within a 2% tolerance range. Berberine was found to have a purity of 99.64%
Conclusion: The study demonstrated that the developed procedure was accurate, precise and reproducible while being easy, environmentally friendly, repeatable, and cost-effective, and it can be used for quantification of berberine in pharmaceutical dosage forms.
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