PHYTOCHEMICAL ANALYSIS AND AN IN VITRO ANTIOXIDANT ACTIVITY OF GOKSHURADI CHURNA, AN AYURVEDIC POLYHERBAL FORMULATION
Abstract
Introduction: The branch of traditional medicine constitutes Ayurveda, Siddha, Unani and Homeopathy. The knowledge of these traditional systems of medicine with the perspective of safety, efficacy, and quality will helps to the traditional legacy and also to rationalize the use of natural products in healthcare as well.
Aim and Objective: The aim and objective of this study was to evaluate an in vitro antioxidant activity and quantification of phytochemicals of polyherbal drug, Gokshuradi churna (G. churna).
Methods: An aqueous extract of G. churna of 10mg/mL concentration was used for the estimations of total phenols, flavanoids, saponins and tannins. Free radical scavenging activity in terms of DPPH, superoxide and nitric oxide scavenging activity of G. churna was carried out by the standard methods.
Results: In this study the phytoconstituents such as flavonoids (69.11±5.31 mg/g quercetin equivalent), phenols (56.39±4.07 mg/g gallic acid equivalent), saponin (73.43 ± 3.41 mg/g diosgenin equivalent) and tannin (58.11±1.41 mg/g tannic acid equivalent) were noticed in G. churna. Among which saponin and flavonoids showed highest content of 73.43 mg/g and 69.11 mg/g respectively in G. churna. An antioxidant activity was evaluated by determining the DPPH radical scavenging concentration (IC50: 79.47 μg/mL; Standard IC50: 59.84 μg/mL), superoxide radical concentration (IC50: 125.12 μg/mL; Standard IC50: 91.57 μg/mL) and nitric oxide radical concentration (IC50: 244.85 μg/mL; Standard IC50: 264.41 μg/mL) under in vitro conditions.
Conclusion: The present study established that the drug showed an effective IC50 concentration for nitric oxide radical scavenging activity. It also scavenges DPPH and superoxide radical scavenging activity. The antioxidant activity of the drug may be due to the polyherbal ingredients present in it. The antioxidant activity in terms of scavenging nitric oxide radical was significantly higher than the standard ascorbic acid and also its activity was concentration-dependent.
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