• Sadish Kumar Shanmugam Department of Pharmaceutical Chemistry, I.T.S College of Pharmacy, Ghaziabad, Uttar Pradesh, India
  • Monika Singh Assistant Professor, ITS College of Pharmacy, Ghaziabad, Uttar Pradesh, India
  • Deepak Kumar Department of Pharmaceutical Chemistry, I.T.S College of Pharmacy, Ghaziabad, Uttar Pradesh, India
  • Rosaline Mishra Professor, Metro College of Health Sciences & Research, Greater Noida, Uttar Pradesh, India
  • Moumita Barman Department of Pharmaceutics, I.T.S College of Pharmacy, Ghaziabad, Uttar Pradesh, India


Context: Gymnema sylvestre (GS) is a plant species that is mostly found in the tropical regions of Asia, Africa and Australia; it is widespread in India and Sri Lanka. According to various scientific studies, GS contains a chemical constituent that is known to suppress the taste for sugar; this kindled our interest to study the various extracts of GS for their antidiabetic potential.

Aim: The current study emphasizes screening the leaves extracts of Gymnema sylvestre using chloroform, ethyl acetate, and water for their phytoconstituents and evaluating their Antidiabetic property. All the individual concentrates were exposed to subjective qualitative examinations for the distinguishing proof of the phytoconstituents, which was followed by evaluating the effect of different extracts of GS on blood glucose level against Streptozotocin induced Diabetic rats as GS had been reported for its antidiabetic activity.

Material and Methods: The plant material Gymnema sylvestre 1.5 kg was macerated with 3.75 liter of chloroform, ethyl acetate and water for comparative antidiabetic activity. A total of 36 male wistar rats were utilized to induce diabetes by means of intraperitoneal injection with 60 mg/kg STZ. The acute toxicity study adhered to the guidelines set by the Organization for Economic Co-operation and Development (OECD 423, 2001). Throughout a period of 21 days, the animals received various treatments. Glucose levels were measured on day 1, which marked the initiation of any treatment, as well as on the 7th, 14th, and 21st days, utilizing a one-touch glucometer. Hematoxylin and eosin staining were employed for histopathological examination of the pancreas. Statistical analysis was conducted using SPSS software, employing one-way ANOVA followed by Dunnet’s multiple comparisons.

Results: Blood glucose levels in streptozotocin induced diabetic rats fed with different GS extracts decreased to normal levels. When the reduced percentage of blood glucose levels of the various extracts was compared with that of glibenclamide (62%), aqueous extract showed maximal (59%) decline, thus confirming the potentiality of the GS as the most significant antidiabetic agent.

Keywords: Gymnema sylvestre, Anti-diabetic, Streptozotocin, Phytochemical


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Author Biography

Rosaline Mishra, Professor, Metro College of Health Sciences & Research, Greater Noida, Uttar Pradesh, India

With over 11 years of rich Teaching and Research experience from various elite institutions, Dr. Rosaline Mishra is an ambitious and self-driven individual who believes in constantly setting goals and striving towards it. She has completed her Ph.D. from Uttarakhand Technical University established by the Government of Uttarakhand. Having published more than 20 research and review articles in various international and national journals and conferences of repute, she also has two Patents published, one in the Register of Indian Government and another in the Register of South Africa. She is a reviewer of two International journals Indexed in SCI. To keep up with the ever-developing teaching and learning pedagogy she has completed Online Refresher Courses in Pharmacy offered by ARPIT (AICTE) and NPTEL and qualified for a certificate course named “Understanding Research Methods” by the University of London. Dr. Mishra has actively taken part in the AKTU Digital Education organized Massive open online course (MOOC). Delivered video lectures in designated subjects for Dr. A.P.J. Abdul Kalam Technical University (AKTU) and has also received an appreciation certificate from the University for developing e-lectures and sharing with students through the University Portal. She has two years of core research experience at Pharma Industry and BITS Pilani- Hyderabad Campus and is Proficient in handling software related to Drug Designing and Docking such as Pyrx, Molgro, Autodock vina, Pymol, Discovery Studio, sophisticated analytical techniques such as HPLC, UV Spectrophotometer, FTIR, GCMS, etc. General Microbiology techniques and molecular biology techniques etc. and has guided more than 10 M.Pharm and B.Pharm students in their projects. Exploring Marine flora as potential Phytopharmaceuticals and nutraceuticals is her research interest.

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How to Cite
Shanmugam, S., M. Singh, D. Kumar, R. Mishra, and M. Barman. “EXPLORING ANTIDIABETIC POTENTIAL OF GYMNEMA SYLVESTRE”. Journal of Applied Pharmaceutical Sciences and Research, Vol. 6, no. 2, Oct. 2023, pp. 30-35, doi:10.31069/japsr.v6i2.06.
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